Non-effect of uremia on analytical recovery of aluminum in serum.

rosine B, Tartrazine, Lissamine Green B, Pyronin G, Acetyl Yellow G, Ponceau 6R, Ponceau SX, Food Orange #2, and Food Yellow #3. The concentration of dye tested was 50 mg/ L of incubation mixture (25 pg/tube) in RIA systems for human lutropin (hLH), its alpha and beta subunits, rat LII, rat corticotropin (rACTH), rat insulin, and human thyroxin (T4) and testosterone. (For T4 we usedthe MAGIC-T4 RIAkit from Corning, Medfleld, MA 02052.) The testosterone RIA involved 3H-tracer and dextran-coated charcoal separation; all others had ‘I-labeled tracers and double-antibody solid-phase separation. All dyes gave strongly colored incubation mixtures at 50 mg/L; most were clearly visible at 10-20 mgfL. For most of the RIA systems, most of dyes could be added to tracer and (or) antibody without substantial effects. Erythrosine B, however, affected not only the initial binding but alsothe nonspecific binding in most of the systems. Also, Lissamine Green B and Pyronin G decreased the initial binding in the testosterone RIA, and Food Orange #2 did the same in the RIA of insulin. In our laboratory we successfully use Pyronin G (10 pg/ tube) as an additive for the tracers, in combination with other dyes for the antisera.